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soluble icam 1  (R&D Systems)


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    R&D Systems soluble icam 1
    Soluble Icam 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/soluble+recombinant+human+icam+1/Recombinant+Human+ICAM-1%2FCD54+Protein%2C+CF/pmc08684734__mbc___32___1749___s001-11-6-10
    Average 94 stars, based on 59 article reviews
    soluble icam 1 - by Bioz Stars, 2026-10
    94/100 stars

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    Article Title: Prolactin stimulates integrin-mediated adhesion of circulating mononuclear cells to endothelial cells.
    Article Snippet: Na51CrO4 was from Amersham Life Science (Buckinghamshire, UK). .. Soluble recombinant human ICAM-1, anti-LFA-1 (BCA1), and anti-VLA-4 (BBA37) mAb were from R&D Systems (Minneapolis, MN, USA). .. Anti-phosphotyrosine (4G10) and anti-paxillin (5H11) mAb were from Upstate Biotechnology (Lake Placid, NY, USA) and polyclonal Ab anti-Jak2, anti-STAT-3 and anti-STAT-5 were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Article Title: Activation of Epac stimulates integrin-dependent homing of progenitor cells.
    Article Snippet: http://bloodjournal.hematologylibrary.org/content/111/5/2640.full.html Updated information and services can be found at: (2497 articles) Hemostasis, Thrombosis, and Vascular Biology Articles on similar topics can be found in the following Blood collections http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about ordering reprints may be found online at: http://bloodjournal.hematologylibrary.org/site/subscriptions/index.xhtml Information about subscriptions and ASH membership may be found online at:



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    TRAF4-NOX complex induced ICAM1 expression through the NF-κB pathway in response to radiation. ( A ) The effects of knockdown of TRAF4, NOX2, or NOX4 on activation of NF-κB signaling were detected by Western blotting. Cell lysates were fractionated into cytosolic extract (CE) and nuclear extract (NE). Tubulin and Lamin A/C were used as markers for CE and NE, respectively. ( B ) Effects of knockdown of TRAF4, NOX2, or NOX4 on transcriptional activity of NF-κB were measured by a luciferase assay. Error bars, ± SD (n = 3); * p < 0.05 compared with non-transfected and non-irradiated cells, ** p < 0.05 compared with irradiated cells. ( C ) Effects of knockdown of TRAF4, NOX2, or NOX4 on radiation-induced mRNA expression of ICAM1 were assessed by qRT-PCR. Error bars, ± SEM (n = 3); * p < 0.05 compared with non-irradiated cells, ** p < 0.05 compared with irradiated cells. ( D ) Effects of knockdown of TRAF4, NOX2, or NOX4 on secretion of sICAM1 were measured by ELISA in CM of normal lung fibroblasts. Error bars, ± SEM (n = 3); * p < 0.05 compared with non-irradiated cells, ** p < 0.05 compared with irradiated cells.

    Journal: Scientific Reports

    Article Title: TRAF4 promotes lung cancer aggressiveness by modulating tumor microenvironment in normal fibroblasts

    doi: 10.1038/s41598-017-09447-z

    Figure Lengend Snippet: TRAF4-NOX complex induced ICAM1 expression through the NF-κB pathway in response to radiation. ( A ) The effects of knockdown of TRAF4, NOX2, or NOX4 on activation of NF-κB signaling were detected by Western blotting. Cell lysates were fractionated into cytosolic extract (CE) and nuclear extract (NE). Tubulin and Lamin A/C were used as markers for CE and NE, respectively. ( B ) Effects of knockdown of TRAF4, NOX2, or NOX4 on transcriptional activity of NF-κB were measured by a luciferase assay. Error bars, ± SD (n = 3); * p < 0.05 compared with non-transfected and non-irradiated cells, ** p < 0.05 compared with irradiated cells. ( C ) Effects of knockdown of TRAF4, NOX2, or NOX4 on radiation-induced mRNA expression of ICAM1 were assessed by qRT-PCR. Error bars, ± SEM (n = 3); * p < 0.05 compared with non-irradiated cells, ** p < 0.05 compared with irradiated cells. ( D ) Effects of knockdown of TRAF4, NOX2, or NOX4 on secretion of sICAM1 were measured by ELISA in CM of normal lung fibroblasts. Error bars, ± SEM (n = 3); * p < 0.05 compared with non-irradiated cells, ** p < 0.05 compared with irradiated cells.

    Article Snippet: Recombinant soluble ICAM1 (ADP4-050) was purchased from R&D system (Minneapolis, MN, USA).

    Techniques: Expressing, Knockdown, Activation Assay, Western Blot, Activity Assay, Luciferase, Transfection, Irradiation, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    CM from TRAF4-knockdown fibroblasts suppressed in vivo tumor progression of NSCLC in a mouse xenograft model. ( A ) Schematic description for generation of mouse xenograft model and treatment of CM. Radiation exposure was locally treated to xenografted tumor sites and each CM was treated for every 4 d, or rICAM1 three times weekly. ( B ) Effects of CM on tumor growth were measured in a mouse xenograft model. Error bars, ± SEM (n = 3); * p < 0.05 compared with irradiated tumor volume. ( C ) In vivo effects of CM on EMT marker of tumor were assessed by Western blotting. ( D ) A schematic diagram illustrates how radiation-induced TRAF4 in fibroblasts promotes lung cancer progression. TRAF4 stabilizes NOX complex and increases secretion of sICAM1 which induces EMT and radioresistance of lung cancer cells.

    Journal: Scientific Reports

    Article Title: TRAF4 promotes lung cancer aggressiveness by modulating tumor microenvironment in normal fibroblasts

    doi: 10.1038/s41598-017-09447-z

    Figure Lengend Snippet: CM from TRAF4-knockdown fibroblasts suppressed in vivo tumor progression of NSCLC in a mouse xenograft model. ( A ) Schematic description for generation of mouse xenograft model and treatment of CM. Radiation exposure was locally treated to xenografted tumor sites and each CM was treated for every 4 d, or rICAM1 three times weekly. ( B ) Effects of CM on tumor growth were measured in a mouse xenograft model. Error bars, ± SEM (n = 3); * p < 0.05 compared with irradiated tumor volume. ( C ) In vivo effects of CM on EMT marker of tumor were assessed by Western blotting. ( D ) A schematic diagram illustrates how radiation-induced TRAF4 in fibroblasts promotes lung cancer progression. TRAF4 stabilizes NOX complex and increases secretion of sICAM1 which induces EMT and radioresistance of lung cancer cells.

    Article Snippet: Recombinant soluble ICAM1 (ADP4-050) was purchased from R&D system (Minneapolis, MN, USA).

    Techniques: Knockdown, In Vivo, Irradiation, Marker, Western Blot